Kevin Gibbons " Convenient, good communication. I received the cards just a few days after ordering. They were exactly as described. Very easy to use. We had taken a sample to our county health dept for testing a couple weeks ago. It came back P for present for e Coli. So we used the R card test. We did not see any colonies on the cards. We will take a new sample to the county to see if they test it positive again or not. The only thing that’s challenging with the r-cards is that we are just doing it at home. No incubator. So we can’t be sure that the cards would grow out anything without the proper temp regulation. The website does not address this."
Thomas Tornabene " Jury is still out. I feel uncomfortable storing the ECC units at room temperature (cool place in a desiccator?). Also concerned about incubation at 37 C for 24 hrs (concern that my self built incubator has a +/- of 4 degrees (set to 33 C) is not suitable to maintain max growth temperature and that the 24 hrs of incubation should be extended but concerned that I could be producing false negatives. Also, the ECC disc warp when removed from the pouch making it more difficult to inoculate but flattens out in the incubator. I need more time to get comfortable with this rapid assay system. I had been using the 3M ECC disk and liked the detection of E. coli by both color and gas production."
R-Card® E.Coli + Coliform Water Testing Kit
G L Alcock Jr" Impressive results and ease of use. We were looking for a system that would point out “hot spots of pollution” in a marina in Florida. This test did just that. We were also surprised how economical it was. Thank you"
David Kutchinski" Wonderful alternative to Colorado State cubicle queens. My fellow vet was told he needed the E. coli and Coliform test to get his loan. Since we was buying a ranch it made no sense. He went to get the test done by the state of Cannabis (aka: Colorado). $300 + three to five weeks for results. Your cards and genius! You include everything down to the pipette! Pop in my microbiology incubator....DING 16 hours later ZERO indication."
Hunter Edward"R-Card for Field Work. Great! Very handy!"
Stella Bowles, MSM, ONS, Canadian environmentalist, Author, Youngest recipient of the Order of Nova Scotia"At the age of 11 I became aware of the pollution of the river running by my house due to the presence of multiple "straight pipes" - sewer pipes running direct from houses, straight into the river. The governments had not done anything to remedy this, until I came along. I set up a science fair project to collect counts of Enterococcus at a number of sites along the river. This would have been impossible if I had had to pay for a commercial lab to do the determinations, but the use of the EasyCard, (now renamed to R-Card), made such "citizen science" both feasible and effective. The first year, my project won in the Regional science fair, but I was too young to be eligible for National competition. So, the following year I extended my methodology, improved my scientific design, and enrolled in the science fair again, (under the project title, "Oh poop, It's Worse than I Thought"). This time I again won regionally, and went on the Nationals, (all of Canada), where I was awarded a Silver Medal. None of this would have been possible without the availability of the EasyCard technology, and the advice of Dr. Roth on its use in our particular circumstances."
Dr. David Maxwell, Retired Doctor, Helped Bowles with her research "The LaHave River, in Nova Scotia, had been documented to have over 650 “straight pipes” – sewage pipes running from houses along the river directly into the river. Governments acknowledged that this practice was illegal, but dismissed repeated calls from a local citizen’s group to enforce the laws, saying that this was not a priority. Enter Stella Bowles, a 14 year old environmental activist who, using the EasyCard, (now renamed as R-Card), technology, sampled the river water at multiple sites, documenting levels of contamination by Enterococci considerably exceeding federal water quality guidelines for even recreational use. As a 14 year old “citizen scientist”, without access to official government-approved laboratories, (which would have cost as much as $4000 in fees), Stella was able to muster local support to cover the minor cost of the cards and supplies from (what is now) Roth Biosciences to do her testing. Armed with her dramatic results, and a highly skilled facility with social media, Stella ultimately shamed three levels of government into crafting a $15 million, multi-year, joint agreement to replace the straight pipes along the Lahave River, incidentally winning multiple awards, including prestigious recognition from the very governments she had lobbied against. Her actions also inspired a YA book, “My River: Cleaning Up the LaHave River”, and led to her instructing other groups of youth in testing their own local environments for bacterial contamination, using the EasyCards. All of this was made possible only by the availability of the cost-effective, and highly user-friendly, card technology from Roth Biosciences. But, not surprisingly, Stella’s campaign provoked a degree of push-back from critics. The first criticism leveled was that the science done by a 14 year old was inherently trivial and invalid. She answered this by citing the advice and approval of her university professor “science advisor” in the design of her project, and the validity of her use of the cards for testing. The next level of criticism was rather more sophisticated, and actually entirely valid, but afforded her an excellent education in the scientific method: How accurate were the counts obtained using the cards? This required two further processes: 1) Running multiple determinations of a single defined sample to determine the reproducibility of the cards, and 2) Running parallel tests on a series of samples, using the EasyCards, matched against determinations by a government accredited lab, as a “gold standard”. This latter was partially hindered by a lack of funding to run large numbers of tests using the commercial lab, coupled with a certain reluctance on the part of the commercial lab itself to disclose the variability of their own determinations. In the end, Stella was able to state that there was a close correlation between the results obtained using the cards and the reference lab, but detailed statistics could not be calculated, due to small numbers and incomplete data from the lab. And this reassurance proved entirely sufficient to silence her critics, within both government and civil society."
Danielle Stewart, RVT Zoo Atlanta "Testing water from our otters' pools for coliforms has been quicker and easier since we switched to R-CARDs. The previous method we used required us to sterilize instruments every two weeks. We also had to purchase expensive broths and mediums. With the R-CARD you don't have to do either so it saves us both time and money. Another reason my team loves the R-CARD is because it's SO MUCH easier to interpret results than the previous method we used. The teal colored dots make colonies obvious and the grid lines make quantifying results straight forward."
R-Card® Total Count
Amber Thomason " Perfect. This is perfect for our homestead, we’re able to test our raw milk using these and our egg incubator. Save money on the lab work! So happy to have found this!"
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