Card Size: 3.5"x 4" Capacity: 1mL
A revolution in bacteria testing, the R-CARD® test method offers the most convenient, streamlined way to detect not only the presence of E. coli and Coliforms, but also the number of colonies. (NOTE: If the sample contains Aeromonas spp., they may give a similar appearance to the true coliform).
Due to variation in the ratios of the glucuronidase and galactosidase enzymes among the many strains of the target organisms (especially E.coli) there may be slight variations in colony color appearances. Each person’s perceptions and interpretations of “blue, magenta, and intermediate” colors may vary and raise interpretation questions. These can be answered definitively by the E. coli/Coliform Verification Kit
- Completely self-contained, requiring almost no additional equipment
- Extremely accurate and easy to understand results
- Fast results in 15-24 hours (incubated)
- Free technical support on any step of the process
- E. coli will show up as dark blue dots, Coliform will show as dark pink/red dots
How It Works
Unlike most E. coli / Coliform media on the market, R-CARD® rapid tests require almost no additional equipment and offer an extremely easy user-experience. Simply lift the R-CARD® ECC clear film and deposit 1 mL of your liquid sample on the card. Then release the clear film so it can fall back on top of the card, covering your sample. Within 1-3 minutes, the liquid sample will solidify between the two layers. This prevents it from dripping or leaking, making R-CARD® rapid tests incredibly easy to transport and use in the field.
For quickest results, it’s best to incubate your sample at 35±0.5°C for 15-24 hours. After the appropriate incubation time, dark blue dots will appear showing E. coli colonies, and dark pink/red colonies will appear showing Coliform colonies. To enumerate, simply count the number of dots for each type.
For more detailed instructions, please check out the PDFs below. Remember, we offer free technical support on any stage of the process and would be happy to instruct and advise you.
Testing 2mL-100mL Aqueous Samples on R-Card® Using Membrane Filter (PDF)
Technical Step-by-Step Instructions: View PDF document
R-CARD General Use Instructions: View PDF document
Additional Helpful and Important Information
Why R-CARD® Rapid Tests Are Better Than Competing Products
R-CARD® rapid tests offer a revolution in convenience without sacrificing accuracy. The cards are completely self-contained and don’t require petri dishes, agar, or other gear that’s fragile and prone to leak. Simply place your sample on the card and wait 1-3 minutes for the liquid sample to solidify between the two layers. This prevents it from dripping or leaking. Cards can easily be stored in a coat or backpack pocket.
It’s also incredibly easy to read the R-CARD’s results. After the appropriate incubation time, simply count the dark blue dots for the number of E. coli colonies and the dark pink/red dots for the number of Coliform colonies. This easy-to-read color system is a significant improvement from other multi-bacteria tests, where differentiating between microbes can be very difficult.
Additional Equipment Needed
While the R-CARD® rapid tests are completely self-contained, you will need a sterile dropper or syringe to dispense your liquid sample onto the card. Once your sample is collected, it’s recommended to use an incubator to rapidly decrease the time it takes to view results (15 to 24 hours with an incubator).
- Food & Beverage
- Environmental Monitoring
- Cosmetics & Personal Care Manufacturing
- Municipal & Environmental Water
Why Choose Roth Bioscience?
Dr. Jonathan Roth is one of the most well-known names in microbiology and bacteria testing. He was the first to develop testing methods to use enzyme substrates for producing different colors to distinguish different types of bacteria. Roth Bioscience is still run by Dr. Roth, who continues to offer some of the most revolutionary innovations in the industry.
Lake Watch of Lake Martin AWW-certified volunteer monitors have been testing sites throughout Lake Martin and its tributaries for the past three decades. We had been using Micrology Laboratories Coliscan Easygel for all of our bacteriological testing until last year. We began encountering lots of defects in materials from Micrology Labs, particularly petri dishes with incomplete coating on the bottom plate (which leads to incomplete gelling of the media when added to the petri dish). So we decided to look for a better alternative. After searching the internet and researching Roth Bioscience’s R-cards, and consulting with our parent organization, Alabama Water Watch, we’re using R-cards for bacteriological sampling. Thus far, here are the advantages we have found with using R-cards versus Coliscan Easygel:
· R-cards do not have the quality assurance issues that Coliscan Easygel has – we were throwing away about 50% of our coliscan petri dishes due to product defects.
· R-cards have a shorter incubation period – can be read in ~ 20 hours, versus 30-48 hours, which is of great importance to our Lake Watch Swim Alert effort (www.lakewatch.org/swim-alert),
· With R-cards, you don’t have to worry about thawing during shipping like Easygel media,
· R-cards are easier to store since they don’t need to be frozen like Easygel media,
· R-cards are easier to use since they don’t require thawing before use and being transported from the sampling site on ice like Easygel media,
· R-cards are easier to read since the colonies appear clearer and easier to distinguish,
· R-cards are easier to read since they have a grid on the cards which makes counting bacteria colonies easier,
· R-cards consistently give results equivalent to Coliscan Easygel, verified through our side-by-side testing.
· You can keep R-cards for weeks to share results visually with others. They are also easy to photograph to share or archive results.
· Lastly, R-cards cost about 25% less than Coliscan Easygel supplies.
Thus, switching to R-cards has been a no-brainer both in terms of ease of conducting water testing and in terms of economics. We are totally pleased with this product and do not hesitate recommending it to other water monitoring groups.
Eric Reutebuch, Former Director, Alabama Water Watch, Former President, Save Our Saugahatchee, Former President, Lake Watch of Lake Martin, current member - Board of Directors, Lake Watch of Lake Martin
I currently utilize Roth Biosciences R card products for E. coil testing at swimming and other recreational use sites in the Choctawhatchee River watershed in SE Alabama where I am the Choctawhatchee Riverkeeper. The products are reliable, produce consistent and accurate results and are reasonably priced. Dr. Roth has produced quality reliable E. coli and coliform test products for 30 years and I have used those products for at least twenty-five years. I started with the Coliscan products from his former company and now I use Roth Biosciences products.
As a volunteer monitor trainer with Alabama Water Watch I have trained teachers, students and citizens to test for E. coli and I still do that but now I train using the R card products. In my personal and professional opinion the R cards offer shorter incubation times, easier enumeration and less mess and waste to dispose.
Before my retirement from Troy University I had students utilize the earlier Coliscan product in their water monitoring studies and presented results at a regional conference. I participated in early tests of handmade prototypes of what are now the Roth Biosciences R card products and recognized the potential for the card-based tests. It pleases me that Dr. Roth developed the R cards and that his company continues to provide quality products, great service and excellent technical advice.
Prior to becoming Choctawhatchee Riverkeeper I did research and public service work at Troy University and served as thesis advisor for several students whose theses involved water quality research. My education includes MS degrees in Chemistry and Biology and certificate programs in Environmental Science and Watershed Management. When I retired and became Choctawhatchee Riverkeeper in 2006 I had thirty years of professional experience most of it in water quality work.