Card Size: 3.5"x 4" Capacity: 1mL
A revolution in E. coli and Coliform testing, the R-CARD® test method offers the most convenient, streamlined way to detect not only the presence of E. coli and Coliform (ECC) in one test, but also the number of colonies. This test medium eliminates Aeromonas false positive as Coliform.
Due to variation in the ratios of the glucuronidase and galactosidase enzymes among the many strains of the target organisms (especially E.coli), there may be slight variations in colony color appearances. Each person’s perceptions and interpretations of “blue, magenta, and intermediate” colors may vary and raise interpretation questions. These can be answered definitively by the E. coli/Coliform Verification Kit
- Completely self-contained, requiring almost no additional equipment
- Extremely accurate and easy to understand results
- Fast results in 15-24 hours (incubated)
- Free technical support on any step of the process
- E. coli will show up as dark blue dots, Coliform will show up as dark pink/red dots
How It Works
Unlike most bacteria testing kits on the market, R-CARD® rapid tests require almost no additional equipment and offer an extremely easy user-experience. Simply lift the R-CARD® clear film and deposit 1 mL of your liquid sample on the card. Then release the clear film so it can fall back on top of the card, covering your sample. Within 1-3 minutes, the liquid sample will solidify between the two layers. This prevents it from dripping or leaking, making R-CARD® rapid tests incredibly easy to transport and use in the field.
For quickest results, it’s best to incubate your sample at 35±0.5°C for 15-24 hours. After the appropriate incubation time, dark blue dots will appear showing E. coli colonies, dark pink/red colonies will appear showing Coliform colonies, and Aeromonas species, if present, are virtually undetectable as they grow as small, light yellow colonies that are very hard to see due to the R-CARD® background. The best way to determine if Aeromonads are present is to do a side-by-side comparison of the test sample on one R-CARD® ECC-A and one R-CARD® ECC and count the Coliform results. If there are more dark pink/red colonies on the standard R-CARD® ECC test card than on the R-CARD® ECC-A, the differential indicates the presence and numbers of Aeromonas in the sample. To enumerate, simply count the number of colonies of each type.
For more detailed instructions, please check out the PDFs below. Remember, we offer free technical support on any stage of the process and would be happy to instruct and advise you.
Testing 2mL-100mL Aqueous Samples on R-Card® Using Membrane Filter (PDF)
Technical Step-by-Step Instructions: View PDF document
R-CARD General Use Instructions: View PDF document
Additional Helpful and Important Information
Why R-CARD® Rapid Tests Are Better Than Competing Products
R-CARD® rapid tests offer a revolution in convenience without sacrificing accuracy. The cards are completely self-contained and don’t require petri dishes, agar, or other gear that’s fragile and prone to leak. Simply place your sample on the card and wait 1-3 minutes for the liquid sample to solidify between the two layers. This prevents it from dripping or leaking. Cards can easily be stored or packed away.
It’s also incredibly easy to read R-CARD® results. After the appropriate incubation time, simply count the dark blue dots for the number of E. coli colonies and the dark pink/red dots for the number of Coliform colonies. This easy-to-read color system is a significant improvement from other multi-bacteria tests, where differentiating between microbes can be very difficult.
Additional Equipment Needed
While the R-CARD® rapid tests are completely self-contained, you will need a sterile dropper or syringe to dispense your liquid sample onto the card. Once your sample is collected, it’s recommended to use an incubator to rapidly decrease the time it takes to view results.
- Food & Beverage
- Environmental Monitoring
- Cosmetic & Personal Care Manufacturing
Why Choose Roth Bioscience?
Dr. Jonathan Roth is one of the most well-known names in microbiology and bacteria testing. He was the first to develop testing methods to use enzyme substrates for producing different colors to distinguish different types of bacteria. Roth Bioscience is still run by Dr. Roth, who continues to offer some of the most revolutionary innovations in the industry.
Choctawhatchee Riverkeeper is fully pleased with the R-card products offered by Roth Biosciences. They are convenient, easy to use, easy to train volunteers to use, produce less waste than alternatives and are relatively inexpensive.
Dr. Roth and others are available and willing to provide technical assistance. This has been the case for myself and subsequently for CRK.