Welcome to Roth Bioscience, LLC formerly known as Micrology Laboratories, LLC

R-CARD® E. coli + Coliform (1 mL) Water Testing Kit
R-CARD® E. coli + Coliform (1 mL) Water Testing Kit
R-CARD® E. coli + Coliform (1 mL) Water Testing Kit
R-CARD® E. coli + Coliform (1 mL) Water Testing Kit
R-CARD® E. coli + Coliform (1 mL) Water Testing Kit
R-CARD® E. coli + Coliform (1 mL) Water Testing Kit

R-CARD® E. coli + Coliform (1 mL) Water Testing Kit

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Card Size: 3.5"x 4"      Capacity: 1mL      STORAGE CONDITIONS AND STORAGE LIFE

A revolution in bacteria testing, the R-CARD® test method offers the most convenient, streamlined way to detect not only the presence of E. coli and Coliforms, but also the number of colonies.(NOTE: If the sample contains Aeromonas spp., they may give a similar appearance to the true coliform). 

Due to variation in the ratios of the glucuronidase and galactosidase enzymes among the many strains of the target organisms (especially E.coli) there may be slight variations in colony color appearances.  Each person’s perceptions and interpretations of “blue, magenta, and intermediate” colors may vary and raise interpretation questions. These can be answered definitively by the E. coli/Coliform Verification Kit

  • 25 tests included in kit
  • Extremely accurate and easy to understand results
  • Results in as little as 24 hours
  • E. coli will show up as dark blue dots, Coliform will appear as dark pink/red dots

Not only does this kit come with R-CARD® ECC rapid tests, it also includes everything you will need to get started testing water.  The kit includes:

  • 25 x R-CARD® ECC (1 mL) test cards
  • 25 x 1 mL Graduated Pipettes, Sterile
  • 25 x 30mL Collection Bottles, Sterile

How It Works

Unlike most E. coli / Coliform media on the market, R-CARD® rapid tests require almost no additional equipment and offer an extremely easy user-experience. Simply lift the R-CARD® ECC clear film and deposit 1 mL of your liquid sample on the card. Then release the clear film so it can fall back on top of the card, covering your sample. Within 1-3 minutes, the liquid sample will solidify between the two layers. This prevents it from dripping or leaking, making R-CARD® rapid tests incredibly easy to transport and use in the field.

For quickest results, it’s best to incubate your sample at 35±0.5°C for 15-24 hours. After the appropriate incubation time, dark blue dots will appear showing E. coli colonies, and dark pink/red colonies will appear showing Coliform colonies. To enumerate, simply count the number of dots for each type.

For more detailed instructions, please check out the PDFs below. Remember, we offer free technical support on any stage of the process and would be happy to instruct and advise you.

Technical Step-by-Step Instructions: View PDF document
R-CARD General Use Instructions: View PDF document
Additional Helpful and Important Information


Why R-CARD® Rapid Tests Are Better Than Competing Products

R-CARD® rapid tests offer a revolution in convenience without sacrificing accuracy. The cards are completely self-contained and don’t require petri dishes, agar, or other gear that’s fragile and prone to leak. Simply place your sample on the card and wait 1-3 minutes for the liquid sample to solidify between the two layers. This prevents it from dripping or leaking. Cards can easily be stored in a coat or backpack pocket.

It’s also incredibly easy to read the R-CARD’s results. After the appropriate incubation time, simply count the dark blue dots for the number of E. coli colonies and the dark pink/red dots for the number of Coliform colonies. This easy-to-read color system is a significant improvement from other multi-bacteria tests, where differentiating between microbes can be very difficult.

Why Choose Roth Bioscience?

Dr. Jonathan Roth is one of the most well-known names in microbiology and bacteria testing. He was the first to develop testing methods to use enzyme substrates for producing different colors to distinguish different types of bacteria. Roth Bioscience is still run by Dr. Roth, who continues to offer some of the most revolutionary innovations in the industry.

Customer Reviews

Based on 5 reviews
Tina Mitchell
6 stars

We have used products from Dr. Roth for decades in teaching settings. They are incredibly easy to use and interpret. These products are Godsend, especially to introductory courses.
I agree with Neal 100% - great customer service. I highly recommend these products...I give 6 stars!

Neal A Meledandri
Great customer service

Customer service is superb. Hard to find good customer service these days. It appears these people actually care about their products and what people think. Pleasure doing business with them. Refreshing change .

Deborah Hatfield
Love R-Card vs Coliscan EZ Gel

This new protocol has saved us significant time and effort and cards are easier to interpret than Coliscan protocol plates we have been using for years. Economics on the cards are good also. Thank you!

G L Alcock Jr
Impressive results and ease of use

We were looking for a system that would point out “hot spots of pollution” in a marina in Florida. This test did just that. We were also surprised how economical it was. Thank you

David Kutchinski
Wonderful alternative to Colorado State cubicle queens

My fellow vet was told he needed the E. coli and Coliform test to get his loan. Since we was buying a ranch it made no sense. He went to get the test done by the state of Cannabis (aka: Colorado). $300 + three to five weeks for results. Your cards and genius! You include everything down to the pipette! Pop in my microbiology incubator....DING 16 hours later ZERO indication.