A revolution in bacteria testing, the R-CARD™ test method offers the most convenient, streamlined way to detect not only the presence of Enterococcus faecalis, but also the number of colonies.
- Completely self-contained, requiring almost no additional equipment
- Extremely accurate and easy to understand results
- Fast results in 14-16 hours (incubated)
- Free technical support on any step of the process
- Enterococcus will show up as teal/green dots
How It Works
Unlike most Enterococcus testing media on the market, R-CARD rapid tests require almost no additional equipment and offer an extremely easy user-experience. Simply lift the R-CARD Enterococcus clear film and deposit 1 mL of your sample on the card. Then release the clear film so it can fall back on top of the card, covering your sample. Within 1-3 minutes, the liquid sample will solidify between the two layers. This prevents it from dripping or leaking, making R-CARD rapid tests incredibly easy to transport and use in the field.
For quickest results, it’s best to incubate your set up test at 35±2°C for 14-16 hours (Enterococci grows quickly in this temperature range). To read your results, simply count the number of teal/green dots which show the number of Enterococcus colonies. Any additional dots that show up beyond 30 hours should not be counted.
For more detailed instructions, please check out the PDFs below. Remember, we offer free technical support on any stage of the process and would be happy to instruct and advise you.
Technical Step-by-Step Instructions: View PDF document
R-CARD General Use Instructions: View PDF document
Why R-CARD™ Rapid Tests Are Better Than Competing Products
R-CARD rapid tests offer a revolution in convenience without sacrificing accuracy. The cards are completely self-contained and don’t require petri dishes, agar, or other gear that’s fragile and prone to leak. Simply place your sample on the card and wait 1-3 minutes for the liquid sample to solidify between the two layers. This prevents it from dripping or leaking. Cards can easily be stored in a coat or backpack pocket.
It’s also incredibly easy to read R-CARD results. They not only detect the presence of Enterococcus, but also the number of colonies. Simply count the number of green/turquoise dots that appear after the appropriate incubation period.
Additional Equipment Needed
While the R-CARD rapid tests are completely self-contained, you will need a sterile dropper or syringe to dispense your liquid sample onto the card. Once your sample is collected, it’s recommended to use an incubator to rapidly decrease the time it takes to view results (15 to 20 hours with an incubator).
- Municipal & Environmental Water
Why Choose Roth Bioscience?
Dr. Jonathan Roth is one of the most well-known names in microbiology and bacteria testing. He was the first to develop testing methods to use enzyme substrates for producing different colors to distinguish different types of bacteria. Roth Bioscience is still run by Dr. Roth, who continues to offer some of the most revolutionary innovations in the industry.