A revolution in Lactic Acid Bacteria testing, the R-CARD™ test method offers the most convenient, streamlined way to detect not only the presence of Lactobacillus spp. but also an accurate count of the number of colonies in as little as 20 hours!
- Completely self-contained, requiring almost no additional equipment
- Extremely Accurate and easy to interpret results
- Fast results start showing in as little as 20 hours (incubated)
- Free technical support on any step of the process
- Lactobacillus spp. will show as green/teal colonies
How It Works
Unlike most Lactic Acid Bacteria testing media on the market, R-CARD rapid tests require almost no additional equipment and offer an extremely easy user-experience. Simply lift the R-CARD Lactic Acid Bacteria clear film and deposit 1 mL of sample on the card. Then release the clear film so it can fall back on top of the card, covering your sample. Within 1-3 minutes, the liquid sample will solidify between the two layers. This prevents it from dripping or leaking, making R-CARD rapid tests incredibly clean and easy to use.
For quickest results, it’s best to incubate your sample at 35±5°C for 20-48hours (Lactobacillus grows quickly in this temperature range). Results will start showing in as little as 20 hours, but we recommend waiting until 24-30 hours to get an accurate count of colonies. To read your results, simply count the number of green/teal dots which are the number of Lactobacillus colonies.
For more detailed instructions, please check out the PDFs below. Remember, we offer free technical support on any stage of the process and would be happy to instruct and advise you.
Technical Step-by-Step Instructions: View PDF document
R-CARD General Use Instructions: View PDF document
Why R-CARD™ Rapid Tests Are Better Than Competing Products
R-CARD rapid tests offer a revolution in convenience without sacrificing accuracy. The cards are completely self-contained and don’t require petri dishes, agar, or other gear that’s fragile and prone to leak. Simply place your sample on the card and wait 1-3 minutes for the liquid sample to solidify between the two layers. This prevents it from dripping or leaking. Cards can easily be stacked in the incubator, reducing the space required for your testing needs.
It’s also incredibly easy to read R-CARD results. They not only detect the presence of Lactobacillus, but also the number of colonies. Simply count the number of green/teal dots that appear after the appropriate incubation period.
Additional Equipment Needed
While the R-CARD rapid tests are completely self-contained, you will need a sterile dropper or syringe to dispense your liquid sample onto the card and a sterile swab to collect the samples to test. Once your sample is collected, it’s recommended to use an incubator to encourage growth, but not required.
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Why Choose Roth Bioscience?
Dr. Jonathan Roth is one of the most well-known names in microbiology and bacteria testing. He was the first to develop testing methods to use enzyme substrates for producing different colors to distinguish different types of bacteria. Roth Bioscience is still run by Dr. Roth, who continues to offer some of the most revolutionary innovations in the industry.