Welcome to Roth Bioscience, LLC formerly known as Micrology Laboratories, LLC


Jonathan N. Roth Ph.D.  June 9, 2022

During the decades since the start of this business, it has evolved through several registered names, including RCR SCIENTIFIC, INC (2001-2006), MICROLOGY LABORATORIES, LLC (2006-2019), AND THE CURRENT NAME OF ROTH BIOSCIENCE, LLC (2019). During this time, Dr. Jonathan N. Roth was a principal and has served as the CEO continuously.  


Prior to, and during this time span, Dr. Roth worked actively in developing, testing and marketing new inventions.  During these activities, he discovered numerous new, previously unknown properties that were released in written form and disclosed as articles in professional journals or included as automatically copyrighted inserts in his web sites. These articles contained useful information available to readers of his web sites and he was happy to make it available.  However, during recent years, it has come to attention that some entities have copied Roth’s work (containing his name as author and originator, and copied it into their own web site or writings as if it were their own work.  This is not only a violation of Roth’s Copyright, but constitutes plagiarism (total dishonesty).  Also, some of his patents have and continue to be violated.

Sometimes, Roth’s work was done in past decades which has been superseded by more and superior inventions, hence Copyright dates of articles on the Roth website may vary from 2006-2022, depending upon when the work and its development took place.  Also, an earlier copyright date may indicate that earlier work may be related to more current work that is an improvement or a superior replacement for the older work. In fact, newer discoveries may or should replace a method described in older work.  In Science, there is always new information that may overshadow previous technology.  Roth Bioscience attempts to include both earlier methods and better recent discoveries. However, the older work may contain results and earlier discovered information that should not be overlooked.  So if one finds plagiarized material, it is wise to go to the original source (the site where it first appeared) to determine if newer and better discoveries are posted.   

Dr. Roth has experienced both violation of his copyrighted work as well as his patents by ambitious corporations, individuals, agencies, and even Professional Organizations who claimed ownership in the face of his previously copyrighted or published patents.  He laments this trend of competition and dishonesty in the Scientific Community.

Last, it should be clearly understood, that neither Roth, nor any of the above mentioned Corporations have ever given permission to any other person or entity to copy or use any of his copyrighted work or plagiarize it.


A number of commercially available products are used to detect various Coliforms via visual indicia (such as discriminating colors detectable under visible and/or ultra violet light). These products are satisfactory for many purposes. However, due to the concurrent presence of non-target micro-organisms, specifically Aeromonas spp, false positive test results can arise when testing for Coliforms with these prior products. Briefly, the false positive test results arise because these “copycatproducts use the same combination of enzyme substrates that were developed and used by a Roth Bioscience predecessor, RCR Scientific, Inc, in the late 1980s. For example, where the galactoside is used as the marker for Coliforms, Aeromonas spp can be present and provide a nearly identical chromogenic pink/red response. This results in the test sample being interpreted to have higher number of Coliforms than is actually the case, a FALSE POSITIVE indication.

R-CARD® ECC-A (patent pending) provides more accurate Coliform detection. Aeromonas species, if present, are initially virtually undetectable, very light yellow colonies that are very hard to detect due to the R-CARD® background. The best way to determine if Aeromonads are present is to do a side-by-side comparison of the test sample on one R-CARD® ECC-A and one R-CARD® ECC and count the Coliform results. If there are more dark pink/red colonies on the standard R-CARD® ECC test card than on the R-CARD® ECC-A, the differential indicates the presence and numbers of Aeromonas in the sample. To enumerate, simply count the number of colonies of each type. Additionally, the pink/red colonies on the R-CARD® can be allowed to mature to the point at which any Aeromonas colonies are visible as faint yellow colonies that can easily be seen and counted.

The R-CARD®ECC-A Medium (patent pending) (ROTH BIOSCIENCE LLC) eliminates the misreading of Aeromonas spp. as Coliforms, an inherent feature of R-CARD®ECC and its numerous “COPYCAT” MEDIA. These “COPYCAT” MEDIA are currently being advertised and sold as specific for the detection and identification of E. coli and Coliform bacteria only. Many of them are also in violation of Patent No. U.S. 8,008.059, and may have been inaccurately approved by officially well-known agencies.